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Title

Ultraviolet-B (UV-B) and ultraviolet-C (UV-C) radiation as elicitors of flavonoid production and effects on genetic stability of Jatropha curcas linn. callus cultures

Author:        

Alvero EMR

Abstract

Jatropha curcas Linn callus cultures were grown in Murashige and Skoog (MS) medium supplemented with napthalene-acetic acid (NAA) and kinetin. The degree of genetic variation in the control cultures and their capacity to produce the flavonoids, apigenin, vitexin and isovitxin were examined through RAPD analysis using 10 arbitrary decamer primers and HPLC, respectively. After 9 weeks in culture and 4 subculture cycles, the calli formed were found to be genetically equivalent to the mother plant indicating that nosomaclonal variation (SV) occurred under such growth conditions. HPLC analysis found that even though vitexin and isovitexin are the dominant flavonoids in the leaves, they were not detected in the callus extracts and despite the absence of detectable levels of apigenin in the leaves, a small amount was found in the callus extracts. These results suggest a correlation between the degree of differentiation and biosynthesis of flavonoids in plant tissues.

Conclusion

Plant local nameTubang-bakod
Scientific nameJatropha curcas
Is local study?Yes
"herbs.ph" plant grouping3
Abstract availabilityYes
"herbs.ph" plant name IDTBB82
CategoryCompleted
Type of studyFor classification
Full text available?
Full citationAlvero EMR. Ultraviolet-B (UV-B) and ultraviolet-C (UV-C) radiation as elicitors of flavonoid production and effects on genetic stability of Jatropha curcas linn. callus cultures [MS thesis] 2010.