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Title

A novel protocol for micropropagation of Rauvolfia serpentina: in low concentration of growth regulators with sucrose and phenolic acid

Author:        

Banerjee M, Modi P

Abstract

A novel in vitro propagation system for Rauvolfia serpentina (Apocynaceae family), a medicinally important plant has been developed. 200 alkaloids have been isolated from the plant. In this study in vitro plantlets were directly regenerated from the apical segments in MS medium supplemented with different concentration of IBA (0.125-0.5 mg/l) in combination with BAP (0.5- 2.0 mg/l), which are far below the limits reported by other workers making this process cost effective. For multiplication of shoots MS media supplemented with BA (0.1 mg/l) and different concentration (0-50 g/l) of sucrose. Use of sucrose especially the higher concentration has never been attempted before and gave very positive results. The elongated and multiple shoot primordial sub-cultured on to rooting media using two-step pulse treatment method using IBA (25-100 μM) and phenolic acid (1%). Rooted plantlets were successfully transferred to the field, after acclimatization in the net house.

Conclusion

Plant local nameSerpentina
Scientific nameRauwolfia serpentina
Is local study?
"herbs.ph" plant grouping3
Abstract availabilityYes
"herbs.ph" plant name IDSPT21
CategoryCompleted
Type of studyFor classification
Full text available?
Full citationBanerjee M, Modi P. A novel protocol for micropropagation of Rauvolfia serpentina: in low concentration of growth regulators with sucrose and phenolic acid. Int J Plant Sci 2010;5(1):93-97.